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US 2020/0347424 A1Published application
Saccharose phosphorylase
AssigneePfeifer & Langen
InventorsTimo Johannes Koch, Thomas Hässler, Birgit Brucher, Andreas Vogel
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AssigneePfeifer & Langen
InventorsTimo Johannes Koch, Thomas Hässler, Birgit Brucher, Andreas Vogel
Estimating the term…
The invention relates to a saccharose phosphorylase that catalyzes the synthesis of glucose-1-phosphate and fructose from saccharose and phosphate, among other things. The saccharose phosphorylase according to the invention can be considered to be a mutation of the saccharose phosphorylase from Bifidobacterium adolescentis. In comparison to wild-type saccharose phosphorylase, the saccharose phosphorylase according to the invention is distinguished by improved activity, process stability, temperature stability, and lower product inhibition and is therefore particularly suitable for use in industrial processes.
A sucrose phosphorylase comprising an amino acid sequence which has an identity in relation to the amino acid sequence according to SEQ ID NO:1 of at least 80% and which, in comparison with SEQ ID NO:1, comprises an amino acid mutation in a sequence segment C) corresponding to positions 175 to 195 according to SEQ ID NO:1;
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Dependencies are inferred from the claim text.