Transgenic plant of the species solanum tuberosum with resistance to phytophthora
AssigneeKWS SAAT SE & Co. KGaA
InventorsJurgen Dietmar STAHL, Nora TEMME
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Abstract
The present invention concerns a transgenic plant of the species Solanum tuberosum with a resistance to an oomycete of the genus Phytophthora, transgenic parts of such a plant, a method for its manufacture and to a composition for external application to plants. On the one hand, nucleotide sequences in accordance with SEQ ID NOS: 1-43 are provided from Phytophthora in a host plant-induced gene silencing strategy in potato plants; on the other hand, a fungicide for plant treatment is provided.
Claims15
1-14. (canceled)
A transgenic plant of the species Solanum tuberosum or a part thereof comprising a stably integrated first double-stranded DNA and a stably integrated second double-stranded DNA, wherein the nucleotide sequences of the coding strands of the first and second DNA are reverse complements of each other, so that a transcript of the first DNA and a transcript of the second DNA are capable of hybridizing to form a double-stranded RNA and whereby the plant expressing said double-stranded RNA is resistant to an oomycete of the genus Phytophthora, wherein the coding strand of the first DNA comprises:
- (a) at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- (b) a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- a nucleotide sequence which hybridizes under stringent conditions to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17 or to a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17.
A method for producing a transgenic plant of the species Solanum tuberosum which plant exhibits a resistance to an oomycete of the genus Phytophthora, comprising the following steps:
- (i) producing a transformed first parent plant comprising a first double-stranded DNA which is stably integrated into the genome of said first parent plant and which has a coding strand which comprises:
- (a) at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- (b) a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- (c) a nucleotide sequence which hybridizes under stringent conditions to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17 or to a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17;
- (ii) producing a transformed second parent plant comprising a second double-stranded DNA which is stably integrated into the genome of said second parent plant, wherein the nucleotide sequences of the coding strands of the first and second DNA are reverse complements of each other, so that a transcript of the first DNA and a transcript of the second DNA are capable of hybridizing to form a double-stranded RNA and whereby the plant expressing said double-stranded RNA becomes resistant to an oomycete of the genus Phytophthora;
- (ii) crossing the first parent plant with the second parent plant;
- and
- selecting a plant in the genome of which both the first double-stranded DNA and the second double-stranded DNA have been stably integrated.
A composition for external application to plants comprising a double-stranded RNA, wherein a strand of said RNA corresponds to a transcript of a double-stranded DNA comprising a coding strand which comprises:
- at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- a nucleotide sequence which hybridizes under stringent conditions to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17 or to a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17.
An isolated nucleic acid sequence comprising a double-stranded first DNA and a double-stranded second DNA, wherein the nucleotide sequences of the coding strands of the first and second DNA are reverse complements of each other, so that a transcript of the first DNA and a transcript of the second DNA are capable of hybridizing to form a double-stranded RNA, wherein the first DNA comprises:
- a. at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- b. a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17, or
- c. a nucleotide sequence which hybridizes under stringent conditions to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17 or to a nucleotide sequence which is complementary to at least 15 successive nucleotides of the nucleotide sequence of SEQ ID NO:
- 17.
Dependencies are inferred from the claim text.