Method for isolation of nucleic acids
OwnerChung-Pei Ouinventor
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Abstract
The current invention provides methods and kits to isolate nucleic acids, including for purification of nucleic acids and nucleic acid enrichment, as well as for identification of nucleic acids in samples where they serve as a biomarker. More specifically, the invention provides a step of binding nucleic acid to a nucleic acid binding molecule which is either (a) a cationic molecule which is an amine, amidine or other amine-derived cation, or (b) a minor groove nucleic acid-binding molecule bound to a solid support and a step of dissociating bound nucleic acids from the nucleic acid-binding molecule by contact with a polyoxometalate or an oxalate.
Claims43
A process for the separation of a nucleic acid from a first nucleic acid-containing composition, comprising the steps of:
- a) providing the first nucleic acid-containing composition in a first phase;
- b) providing a nucleic acid binding molecule which is either (a) a cationic molecule which is an amine, amidine or other amine-derived cation, or (b) a minor groove nucleic acid-binding molecule in a second phase;
- c) allowing contact to be maintained between the first phase and the second phase for a sufficient time for nucleic acids in the first nucleic acid-containing composition to bind to the nucleic acid-binding molecule;
- d) separating the first phase from the second phase;
- and
- e) treating the second phase to dissociate bound nucleic acids from the nucleic acid-binding molecule by contact with a dissociation composition comprising a polyoxometalate or an oxalate molecule to produce a second nucleic acid-containing composition.
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The process of claim 21, wherein the cationic molecule is a cationic polymer selected from polyethyleneimine, poly-lysine, poly-arginine, an amidine-containing polymer, or a guanidino polymer.
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A kit comprising a first composition comprising a nucleic acid binding molecule which is either (a) a cationic molecule which is an amine, amidine or other amine-derived cation, or (b) a minor groove nucleic acid-binding molecule;
- and a second composition comprising a polyoxometalate or an oxalate, wherein addition of the second composition to nucleic acids bound to the nucleic acid binding molecule results in disassociation of the nucleic acids from the nucleic acid binding molecule.
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Dependencies are inferred from the claim text.