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AssigneesDuke University, North Carolina State University
InventorsCharles A. Gersbach, Adrian Pickar Oliver, Rodolphe Barrangou
Estimating the term…
Disclosed herein are Type I Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) programmable systems and methods of using said Type I CRISPR/Cas programmable systems for activating gene expression, repressing gene expression, and gene editing. The disclosure relates to compositions that include Type I CRISPR-Cas fusion proteins designed for transcriptional activation, transcriptional repression, and/or gene editing of target genes in eukaryotic cells. The disclosure relates to Type I Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) transcriptional activation system related compositions and methods of using said Type I CRISPR/Cas transcriptional activation system related compositions for activating gene expression. The disclosure relates to compositions that include a Type I CRISPR-Cas fusion protein designed for transcriptional activation of target genes in eukaryotic cells.
A Type I-E CRISPR-based programmable transcriptional activation (Type I-E CRISPRa) system composition for activating at least one target gene in a eukaryotic cell, the composition comprising at least one polynucleotide sequence encoding:
An expression cassette or a vector comprising at least one polynucleotide sequence of SEQ ID NO:
Dependencies are inferred from the claim text.