Method of selecting a probiotic
AssigneeSociete des Produits Nestle S.A.
InventorsStephane DUBOUX, Marie Noelle HORCAJADA, Laure POQUET
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Abstract
The present invention relates generally to probiotics, in particular probiotics capable of bio-converting olive polyphenols. The invention relates to a method of selecting a probiotic strain or probiotic strain mixture. Further aspects of the invention include the use of a probiotic strain to increase the bio-efficacy of olive polyphenols and a composition comprising a probiotic strain for use in the potentiation of a therapeutic effect and/or a prophylactic effect of oleuropein.
Claims15
Method of selecting a probiotic strain or probiotic strain mixture the method comprising the steps,
- a. identifying a probiotic strain having a β-glucosidase encoding gene by in silico analysis and/or β-glucosidase activity by in vitro analysis of a plurality of strains;
- b. identifying a probiotic strain having an esterase encoding gene by in silico analysis and/or esterase activity by in vitro analysis of a plurality of strains;
- c. fermenting oleuropein with i) a bacterial culture of a probiotic strain identified in step a as having β-glucosidase activity and a probiotic strain identified in step b as having esterase activity, or ii) a bacterial culture of a probiotic strain identified in steps a and b as having both β-glucosidase and esterase activity;
- d. identifying whether the metabolites formed during the fermentation of step c include hydroxytyrosol acetate, hydroxytyrosol or elenolic acid;
- e. measuring the proportion of oleuropein remaining as a function of fermentation time in step c;
- and
- f. selecting a probiotic strain or probiotic strain mixture wherein the probiotic strain or probiotic strain mixture consumes at least 10 wt. % of oleuropein in step c over a time period of less than 24 hours, and wherein the metabolites formed during the fermentation of step c comprise a molecule selected from the group consisting of hydroxytyrosol acetate, hydroxytyrosol and elenolic acid.
A method for increasing the efficacy of olive polyphenols comprising adding to the polyphenols a probiotic strain or probiotic mixture obtained by
- a. identifying a probiotic strain having a β-glucosidase encoding gene by in silico analysis and/or β-glucosidase activity by in vitro analysis of a plurality of strains;
- b. identifying a probiotic strain having an esterase encoding gene by in silico analysis and/or esterase activity by in vitro analysis of a plurality of strains;
- c. fermenting oleuropein with i) a bacterial culture of a probiotic strain identified in step a as having β-glucosidase activity and a probiotic strain identified in step b as having esterase activity, or ii) a bacterial culture of a probiotic strain identified in steps a and b as having both β-glucosidase and esterase activity;
- d. identifying whether the metabolites formed during the fermentation of step c include hydroxytyrosol acetate, hydroxytyrosol or elenolic acid;
- e. measuring the proportion of oleuropein remaining as a function of fermentation time in step c;
- and
- f. selecting a probiotic strain or probiotic strain mixture wherein the probiotic strain or probiotic strain mixture consumes at least 10 wt. % of oleuropein in step c over a time period of less than 24 hours, and wherein the metabolites formed during the fermentation of step c comprise a molecule selected from the group consisting of hydroxytyrosol acetate, hydroxytyrosol and elenolic acid.
Dependencies are inferred from the claim text.