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US 2020/0270681 A1Published application
Methods for analyzing nucleic acids from single cells
Assignee10x Genomics, Inc.
InventorsSydney Brenner, Gi Mikawa, Robert Osborne, Andrew Slatter
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Abstract
Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.
Claims7
(canceled)
A method for biological analysis, the method comprising:
- (a) providing a plurality of polynucleotides derived from a tissue sample;
- (b) generating a plurality of tagged polynucleotides from said plurality of polynucleotides and a plurality of oligonucleotide tags, wherein a tagged polynucleotide of the plurality of tagged polynucleotides comprises:
- (i) a first tag sequence corresponding to a site within said tissue sample;
- and
- (ii) a second tag sequence distinguishing said tagged polynucleotide from other tagged polynucleotides of the plurality of tagged polynucleotides;
- (c) sequencing said plurality of tagged polynucleotides to determine sequences corresponding to the plurality of polynucleotides, including the first tag sequence and the second tag sequence;
- and
- (d) using the sequences determined in step (c) to count polynucleotides for multiple different polynucleotides derived from said tissue sample.
5 more elements
Dependencies are inferred from the claim text.