Glucoamylase blends
AssigneeDanisco US Inc.
InventorsVivek SHARMA, Paula Johanna Maria TEUNISSEN
Life of this patent
Estimating the term…
Abstract
Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of Fusarium verticillioides glucoamylase (Fv-GA) and further contacting the starch substrate with at least one additional glucoamylase. Additional methods are provided for saccharifying and fermenting a starch substrate to produce an end product, a biochemical end product and a fermentable beverage using a combination of Fv-GA and at least one additional glucoamylase.
Claims20
A method for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting of the sequence of Fv-GA (SEQ ID NO:
- 1), having at least 85% identity to the sequence of Fv-GA (SEQ ID NO:
- 1), having at least 85% identity to a catalytic domain of Fv-GA (SEQ ID NO:
- 1), having at least 85% identity to a linker and a catalytic domain of Fv-GA (SEQ ID NO:
- 1) or having at least 85% identity to an active fragment of Fv-GA (SEQ ID NO:
- 1) having glucoamylase activity, and contacting the starch substrate with at least one additional glucoamylase.
17-23. (canceled)
A method for saccharifying and fermenting a starch substrate to produce a biochemical end product, comprising contacting the starch substrate with a glucoamylase having at least 85% identity to Fv-GA (SEQ ID NO:
- 1), and with a glucoamylase having at least 85% identity to Hg-GA (SEQ ID NO:
- 5), under conditions suitable for biochemical fermentation
(canceled)
Dependencies are inferred from the claim text.