Transgenic strain for producing succinate and method for producing succinate
AssigneeNational Chiao Tung University
InventorsEthan I LAN, Martin LAI, Ryan TUNG, Jemmy TSAI, Jason KU
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AssigneeNational Chiao Tung University
InventorsEthan I LAN, Martin LAI, Ryan TUNG, Jemmy TSAI, Jason KU
Estimating the term…
The present invention provides a transgenic strain for producing succinate. The transgenic strain comprises an autotrophic host cell, and a plurality of exogenous genes within the host cell. The exogenous genes include a gene for expressing α-ketoglutarate decarboxylase (Kgd), a gene for expressing succinate semialdehyde dehydrogenase (GabD), a gene for expressing citrate synthase (GltA) and a gene for expressing phosphoenolpyruvate carboxylase (Ppc). Further, expression of at least one of the native genes encoding glucose-1-phosphate adenylyltransferase (GlgC), succinate dehydrogenase subunit A (SdhA), and succinate dehydrogenase subunit B (SdhB) is suppressed in the host cell. The present invention further provides a method for producing succinate, which comprises: providing a transgenic strain of the present invention, and culturing the transgenic strain under a preset condition.
A transgenic strain for producing succinate, comprising:
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