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AssigneeCargill, Incorporated
InventorsCHRISTOPHER K. MILLER, Ana Negrete-Raymond, Brian J. Rush, Amit Vas, Jon Veldhouse
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Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO:44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.
A fermentation method comprising:
The fermentation method of any one of the previous claims wherein the genetically modified yeast is prepared from a yeast that does not have a sugar transporter with 90% or greater identity to Sc MAL11 (SEQ ID NO:7), a functional isomaltose transporter, or from a yeast that has no ability to grow on maltulose.
The fermentation method of any one of the previous claims wherein the genetically modified yeast is Saccharomyces cerevisiae.
The fermentation method of any one of the previous claims wherein the genetically modified yeast comprises a heterologous polypeptide having 90% or greater identity to SEQ ID NO:44.
The fermentation method of any one of the previous claims wherein at the first time point isomaltose is in an amount in the range of 0.25-5% (wt) of the fermentable carbohydrates.
The fermentation method of any of the previous claims wherein the concentration of maltulose at the second time point is not more than four times the concentration at the first time point.
The fermentation method of claim 11 wherein the concentration of maltulose at the second time point not more than is two times the concentration at the first time point.
The fermentation method of any of the previous claims wherein the genetically modified yeast produce a bioproduct.
The fermentation method of any one of the previous claims wherein genetically modified yeast further comprises a heterologous isomaltase, or an endogenous isomaltase expressed at levels higher than in an unmodified yeast.
The fermentation method of any one of the previous claims wherein the genetically modified yeast further comprises a heterologous starch-degrading enzyme.
A fermentation method comprising:
A genetically modified yeast comprising a heterologous di- or tri-glucopyranosyl sugar transporter, wherein the yeast is capable of growing at a rate of 0.02 or greater on synthetic media comprising a carbohydrate composition wherein maltulose is present in an amount of at least 97% (wt) in the carbohydrate composition, grown under standard conditions.
A genetically modified Crabtree-positive yeast comprising a heterologous polypeptide having 90% or greater identity to SEQ ID NO:44.
A nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide having 90% or greater identity to SEQ ID NO:44 and one or more heterologous nucleic acid regulatory sequence(s).
A nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide having 90% or greater identity to SEQ ID NO:44 and a nucleic acid sequence from Saccharomyces cerevisiae.
A vector comprising a nucleic acid encoding a polypeptide having 90% or greater identity to SEQ ID NO:44.
A genetically modified Saccharomyces cerevisiae-positive yeast comprising
A fermentation method comprising:
Dependencies are inferred from the claim text.