Broad Resistance to Soybean Cyst Nematode
AssigneesBoard of Trustees of Southern Illinois University, The Curators of the University of Missouri
InventorsKhalid Meksem, Henry Nguyen, Naoufal Lakhssassi, Mariola Klepadlo, Gunvant Patil
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Abstract
A transgenic soybean plant resistant to soybean cyst nematode (SCN), or parts thereof, are provided. Also provided are methods of increasing SCN resistance of a soybean plant and associated DNA constructs.
Claims21
A plant of an agronomically elite soybean variety, comprising a first polynucleotide encoding a serine hydroxymethyltransferase promoter that functions in the soybean plant operably linked to a second polynucleotide encoding a polypeptide having serine hydroxymethyltransferase activity;
- wherein said first polynucleotide comprises SEQ ID NO:
- 1, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof;
- wherein said first polynucleotide further comprises one or more mutations of SEQ ID NO:
- 1 selected from the group consisting of:
- A3959T, G3726C, A3444T, C3147T, A3130C, T3037C, G2999C, C2998T, T2979C, C2846T, G2475T, A2420G, C2416T, +2323T, T2051A, G2050C, A1606G, T1523-, G1164A, T1156A, A403C, C380T, A338T, T329A, T313C, T225G, T225-, A133G, A133-, G28T, and G28-;
- and
- wherein the plant has increased soybean cyst nematode (SCN) resistance compared to a control soybean plant lacking said first polynucleotide.
A plant of an agronomically elite soybean variety, comprising a first polynucleotide encoding a serine hydroxymethyltransferase promoter that functions in the soybean plant operably linked to a second polynucleotide encoding a polypeptide having serine hydroxymethyltransferase activity;
- wherein said polypeptide having serine hydroxymethyltransferase activity comprises SEQ ID NO:
- 2, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof;
- wherein said polypeptide having serine hydroxymethyltransferase activity further comprises one or more mutations of SEQ ID NO:
- 2 selected from the group consisting of:
- I107F, P200R, P200-, N459Y, and N459H;
- wherein the plant has increased soybean cyst nematode (SCN) resistance compared to a control soybean plant lacking said second polynucleotide;
- and
- wherein said second polynucleotide has increased expression, an altered expression pattern, or an increased copy number.
A DNA construct comprising a first polynucleotide encoding a serine hydroxymethyltransferase promoter that functions in a soybean plant operably linked to a second polynucleotide encoding a polypeptide having serine hydroxymethyltransferase activity;
- wherein said first polynucleotide comprises SEQ ID NO:
- 1, or a sequence at least 95% identical thereto, or a full-length complement thereof, or a functional fragment thereof;
- and
- wherein said first polynucleotide further comprises one or more mutations of SEQ ID NO:
- 1 selected from the group consisting of:
- A3959T, G3726C, A3444T, C3147T, A3130C, T3037C, G2999C, C2998T, T2979C, C2846T, G2475T, A2420G, C2416T, +2323T, T2051A, G2050C, A1606G, T1523-, G1164A, T1156A, A403C, C380T, A338T, T329A, T313C, T225G, T225-, A133G, A133-, G28T, and G28-.
Dependencies are inferred from the claim text.