Stem Rust Resistance Gene
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsSambasivam Kuppusamy Periyannan, Peter Norman Dodds, Rohit Mago, Evans Lagudah
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Abstract
The present invention relates to a transgenic plant which integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to one or more races of Puccinia graminis f. sp. tritici, such as the Ug99 group of races Puccinia graminis f. sp. tritici.
Claims34
A plant genetically modified to comprise a gene encoding an Sr50 polypeptide which comprises amino acids having a sequence as provided in SEQ ID NO:1 or an amino acid sequence which is at least 82% identical to SEQ ID NO:1, wherein the polypeptide confers resistance to one or more races Puccinia graminis.
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A process for identifying a polynucleotide encoding a polypeptide which confers resistance to Puccinia graminis comprising:
- i) obtaining a polynucleotide operably linked to a promoter, the polynucleotide encoding a polypeptide comprising amino acids having a sequence as provided in SEQ ID NO:1, or an amino acid sequence which is at least 82% identical to SEQ ID NO:1,
- ii) introducing the polynucleotide into a plant,
- iii) determining whether the level of resistance to Puccinia graminis is modified relative to an isogenic plant lacking the polynucleotide, and
- iv) optionally, selecting a polynucleotide which when expressed confers resistance to Puccinia graminis.
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A chimeric vector comprising a polynucleotide comprising nucleotides having a sequence as provided in SEQ ID NO:10, a sequence which is at least 82% identical to SEQ ID NO:10, or a sequence which hybridizes to SEQ ID NO:10, wherein the polynucleotide is operably linked to a promoter.
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A method of producing a plant which has integrated into its genome a gene encoding an Sr50 polypeptide which comprises amino acids having a sequence as provided in SEQ ID NO:1 or an amino acid sequence which is at least 82% identical to SEQ ID NO:1, wherein the polypeptide confers resistance to one or more races Puccinia graminis, the method comprising the steps of
- i) crossing two parental plants, wherein at least one plant comprises a gene encoding an Sr50 polypeptide which comprises amino acids having a sequence as provided in SEQ ID NO:1 or an amino acid sequence which is at least 82% identical to SEQ ID NO:1, wherein the polypeptide confers resistance to one or more races Puccinia graminis,
- ii) screening one or more progeny plants from the cross for the presence or absence of the gene, and
- iii) selecting a progeny plant which comprise the gene,
- thereby producing the plant.
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Use of the polynucleotide comprising nucleotides having a sequence as provided in SEQ ID NO:10, a sequence which is at least 82% identical to SEQ ID NO:10, or a sequence which hybridizes to SEQ ID NO:10, to produce a recombinant cell and/or a transgenic plant.
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A method for identifying a plant comprising a polynucleotide encoding an Sr50 polypeptide which confers resistance to Puccinia graminis, the method comprising the steps of
- i) obtaining a nucleic acid sample from a plant, and
- ii) screening the sample for the presence or absence of the polynucleotide,
- wherein presence of the polynucleotide indicates that the plant is resistant to Puccinia graminis.
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Flour or wholemeal comprising a gene encoding an Sr50 polypeptide which comprises amino acids having a sequence as provided in SEQ ID NO:1 or an amino acid sequence which is at least 82% identical to SEQ ID NO:1, wherein the polypeptide confers resistance to one or more races Puccinia graminis.
Dependencies are inferred from the claim text.