Plants having increased tolerance to herbicides
AssigneeBASF Agro B.V.
InventorsRaphael Aponte, Stefan Tresch, Dario Massa, Matthias Witschel, Tobias Seiser, Jill Marie Paulik
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Abstract
The present invention refers to a plant or plant part comprising a polynucleotide encoding a mutated PPO polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to herbicides.
Claims32
A plant or plant part comprising a polynucleotide encoding a mutated PPO polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to herbicides, wherein said mutated PPO polypeptide comprises one or more of the following motifs. 1) Motif 1:
- GT[C/S]GGDP (SEQ ID NO:
- 131)
- Wherein the glycine at position 4, and/or 5 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
- 2) Motif 2:
- [A/S/C]PS[D/N][X][X]L (SEQ ID NO:
- 132)
- Wherein the serine at position 3 within said motif of the corresponding wildtype sequence is substituted by any other amino acid 3) Motif 3:
- [R/Q][E/D]KQQ[L/Y]P (SEQ ID NO:
- 133)
- Wherein the glutamine at position 4 within said motif of the corresponding wildtype sequence is substituted by any other amino acid
- 4) Motif 4:
- L[I/V]PSKE (SEQ ID NO:
- 134)
- wherein the serine at position 4 within said motif of the corresponding wildtype sequence is substituted by any other amino acid.
An isolated and/or recombinantly produced and/or synthetic nucleic acid molecule comprising a nucleic acid molecule encoding a mutated PPO polypeptide selected from the group consisting of:
- (a) a nucleic acid molecule encoding a mutated PPO polypeptide comprising the sequence of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117 or 129, or a variant, paralogue, orthologue or homolog thereof;
- (b) a nucleic acid molecule, which, as a result of the degeneracy of the genetic code, can be derived from a nucleic acid molecule encoding a mutated PPO polypeptide comprising the sequence of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117 or 129, or a variant, paralogue, orthologue or homolog thereof, and confers increased herbicide tolerance or resistance, as compared to a corresponding, non-transformed, wild type plant cell, a plant or a part thereof;
- (c) a nucleic acid molecule encoding a mutated PPO polypeptide having 30% or more identity with the amino acid sequence of the PPO polypeptide sequence of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117 or 129, and confers increased herbicide tolerance or resistance, as compared to a corresponding, non-transformed, wild type plant cell, a plant or a part thereof;
- (d) nucleic acid molecule which hybridizes with a nucleic acid molecule of (a), (b), or (c), under stringent hybridization conditions and confers increased herbicide tolerance or resistance, as compared to a corresponding, non-transformed, wild type plant cell, a plant or a part thereof;
- wherein the amino acid sequence of the encoded mutated PPO polypeptide differs from the wildtype amino acid sequence of a PPO polypeptide at one or more positions corresponding to positions 32, 53, 57, 61, 63, 64, 65, 67, 71, 76, 82, 83, 85, 86, 87, 88, 91, 103, 104, 106, 108, 116, 119, 126, 127, 129, 139, 159, 210, 211, 224, 245, 246, 248, 249, 252, 253, 254, 255, 257, 259, 260, 262, 264, 286, 291, 305, 308, 309, 323, 335, 343, 345, 358, 372, 373, 387, 391, 392, 400, 412, 414, 415, 425, 428, 431, 433, 434, 435, 436, 447, 451, 453, 464, 466, 482 of SEQ ID NO:
- 1 or 2, wherein said difference refers to a substitution of the amino acid at that positions by any other amino acid.
A plant product obtained from a plant or plant part thereof, wherein the plant or plant part comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated PPO polypeptide encoded by the polynucleotide, the expression of the mutated PPO polypeptide conferring to the plant tolerance to herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait.
Dependencies are inferred from the claim text.