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AssigneeRothamsted Research Limited
InventorsKonstantin Kanyuka, Kim E. Hammond-Kosack, Clement Bouton
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The invention relates to the field of genetic engineering tools for gene expression in plants. Specifically, the invention concerns modified Foxtail Mosaic Virus (FoMV) vectors comprising polynucleotide sequences which are capable of driving expression of a gene of interest in a plant host. Accordingly, the invention concerns FoMV-based expression vectors comprising said polynucleotides, compositions comprising modified FoMV vectors, methods of generating gene expression in plants infected with the modified FoMV vectors. The expression vectors, compositions, plants and methods of the present invention find application in many fields of biotechnology, including, for example, gene characterization, protein production and agricultural biotechnology.
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A modified Foxtail Mosaic Virus (FoMV) for expression of a gene of interest (GOI) in a plant, plant part or plant cell, wherein the virus comprises a first sgp2 promoter and at least a second sgp2 promoter;
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A Foxtail Mosaic Virus (FoMV) DNA expression construct comprising from 5′ to 3′ polynucleotide sequences encoding a strong heterologous promoter that is active in plants, followed by the viral ORF1, sgp1 promoter, ORF2, ORF3, ORF 4, at least two sgp2 promoters, coat protein (CP) and nopaline synthase terminator (nos), wherein the ORF2 overlaps with ORF3 and ORF3 overlaps with ORF4, and ORF4 includes the start codon of ORF5A, wherein the polynucleotide sequence comprised in or between the duplicated sgp2 promoters includes insertion site(s) for a gene of interest (GOI).
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Dependencies are inferred from the claim text.