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US 2020/0270599 A1Published application
Nanocaged enzymes with enhanced catalytic activity and increased stability
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsJinglin FU, Zhao ZHAO, Neal WOODBURY, Hao YAN
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Abstract
The present disclosure describes a nanoparticle comprising a three dimensional DNA nanocage and a payload biological macromolecule, and methods of assembly thereof.
Claims20
A nanocage comprising;
- a plurality of structural members comprising a single-stranded circular DNA sequence selected from p7308, p7560, p7704, p8064, p8634, and pEGFP, wherein internal surfaces of the plurality of structural members form an inner cavity, wherein architectural arrangement of the structural members in the three dimensional body forms an arrangement selected from the group consisting of a honeycomb lattice, a single-walled square lattice, and a double-walled square lattice.
A nanoparticle comprising:
- a nanocage comprising a plurality of structural members comprising DNA in a three-dimensional lattice, wherein internal surfaces of the plurality of structural members form an inner cavity;
- and
- one or more payload molecules bound to internal surfaces of the inner cavity, wherein the one or more payload molecules comprise enzymes, nucleic acids, polypeptides, antibodies, phospholipids, or any combination thereof;
- wherein a first structural member of the plurality of structural members is linked to a second structural member of the plurality of structural members by short bridge DNA strands,
- wherein said enzymes, polypeptides, or antibodies comprise a hexahistidine sequence or a lysine residue.
2 more elements
A method of making a nanoparticle comprising, the method comprising;
- trapping a payload macromolecule in an open half cage comprising a single-stranded circular DNA sequence selected from p7308, p7560, p7704, p8064, p8634, and pEGFP;
- and
- assembling two of said half cages into a closed nanocage having an inner cavity and nanopores;
- wherein the inner cavity comprises the payload macromolecule.
1 more element
A nanoparticle comprising:
- a nanocage comprising a plurality of structural members comprising DNA in a three-dimensional lattice, wherein internal surfaces of the plurality of structural members form an inner cavity;
- and one or more payload molecules bound to internal surfaces of the inner cavity, wherein the one or more payload molecules comprise enzymes, nucleic acids, polypeptides, antibodies, phospholipids, or any combination thereof;
- wherein a first structural member of the plurality of structural members is linked to a second structural member of the plurality of structural members by short bridge DNA strands.
Dependencies are inferred from the claim text.