US 2020/0270295 A1 · N4-MODIFIED CYTIDINE NUCLEOTIDES AND THEIR USE

Abstract

Disclosed are W-position modified cytidine nucleotides of formula (I). Provided herein are methods of chemical synthesis of AP-modified cytidine nucleoside triphosphates and their applications as well as uses of the cytidine analogues for the synthesis of modified nucleic acids. The nucleic acid molecule includes DNA, RNA or a combination of DNA/RNA. One of many applications of modified cytidine nucleotides described herein is enzyme selection, when an enzyme of interest bears an activity of an esterase, amidase, oxidoreductase, lyase, ligase or other enzymatic activity, formula (I) wherein the substituants are as defined in the appended claims.

Claims19

Dependent claims

A compound of structural Formula I:

wherein
R is independently selected from the group consisting of:
—(CH2)n—CH3, wherein n is 4, 5, 6, 7, 8, 9 or 10;
wherein * is the point of attachment of the R group;
wherein R′ is independently selected from the group consisting of:
wherein n is 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10;
and wherein * is the point of attachment of the R′ group to the R group;
R″ is independently selected from the group consisting of —H, —CH3, —NH2, —OH, —Cl, and —Br;
R1 is independently selected from the group consisting of —H, —OAc, —OBz, —Me, and —Et;
R2 is independently selected from the group consisting of —H, —OH, —OMe, and —OEt.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/646,593Filed 12 Sep 2018

Priority

LT LT2017 52312 Sep 2017

Published

27 Aug 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C07H 19/10
Pyrimidine radicals: with the saccharide radical esterified by phosphoric or polyphosphoric acids
C07H 21/04
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of…: with…
C12N 15/1075
Isolating an individual clone by screening libraries: by coupling phenotype to genotype, not provided for in other groups of this subclass
C12Q 1/68
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…

N4-modified cytidine nucleotides and their use

Life of this patent

N4-modified cytidine nucleotides and their use

Life of this patent

Abstract

Claims19