Chromatographic seal and coated flow paths for minimizing analyte adsorption

A chromatographic column for separating analytes in a sample, the chromatographic column comprising:

• a column body having an interior surface, at least a portion of the interior surface coated with an alkylsilyl coating;
• and
• a filter configured to connect to the column, the filter comprising
• a porous element, at least a portion of the porous element coated with the alkylsilyl coating;
• a compression element configured to receive the porous element thereby forming an assembly;
• and
• a housing having an opening formed therein, the opening configured to receive the assembly,
• wherein the assembly is retained within the opening when the assembly is received therein;
• and
• wherein the alkylsilyl coating is undamaged when the filter is connected to the column body.