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US 2020/0268804 A1Published application
Methods and compositions to maintain stem cell quiescence
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsMarco Quarta, Thomas A. Rando
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Abstract
Compositions and methods are provided for induction and maintenance of quiescence of stem cells.
Claims22
A composition, comprising:
- four or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, SB203580, SU5402, TGFβ, and a serum replacement.
A composition, comprising:
- four or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, MGCD-265, JNJ-7706621, SB203580, SU5402, somatostatin, TGFβ, and a serum replacement.
A composition, comprising:
- an isolated population of stem cells in a culture medium, the culture medium comprising four or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, SB203580, SU5402, TGFβ, and a serum replacement.
A method of culturing stem cells, comprising:
- culturing ex vivo an isolated population of stem cells in a medium comprising four or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, MGCD-265, JNJ-7706621, SB203580, SU5402, somatostatin, TGFβ, and a serum replacement for a period of at least 6 hours.
A method of inducing or maintaining quiescence of an isolated population of stem cells, comprising:
- culturing ex vivo an isolated population of stem cells in a medium comprising four or more components selected from the group consisting of elcatonin, forskolin, insulin-transferrin-selenium, MGCD-265, JNJ-7706621, SB203580, SU5402, somatostatin, TGFβ, and a serum replacement for a period of at least 6 hours.
Dependencies are inferred from the claim text.