Identification of unknown compounds by using a novel retention index system in liquid chromatography
AssigneeChromaleont Srl
InventorsLuigi MONDELLO, Francesca RIGANO
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Abstract
A method for the identification of unknown compounds based on a novel Retention Index System comprising having a TAGs homologous series, wherein such identification is performed by means of liquid chromatography (LC), or liquid chromatography coupled with mass spectrometry (LC-MS) is disclosed.
Claims17
(canceled)
The method of claim 4, wherein the homologous series comprises triacylglycerols ranging from triheptanoin to triheneicosanoin, or any portion thereof.
A method for the identification of unknown compounds in liquid chromatography based on a homologous series of triacylglycerols (TAGs) composed by odd-chain fatty acids as Retention Index System comprising the following steps:
- a. attribution of a retention index value to each reference TAG;
- b. chromatographic separation of a TAGs reference mixture;
- c. chromatographic separation of unknown compounds in a sample;
- d. determination of the retention index value of each separated unknown compound;
- e. identification of the unknown compound.
A kit for use as retention index system in liquid chromatography comprising a homologous series of triacylglycerols composed by odd-chain fatty acids.
Dependencies are inferred from the claim text.