Fixation and retention of extracellular vesicles
AssigneeCornell University
InventorJohn T. G. PENA
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Abstract
The present invention relates to a method of fixing extracellular vesicles. The method includes providing a sample containing extracellular vesicles and contacting the sample with a non-reversible cross-linking agent and, optionally, an aldehyde-containing fixative. Preferably the non-reversible cross-linking agent is 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. The method also includes imaging the fixed extracellular vesicles for the determination or exclusion of disease or disorder in a clinical sample. The present invention also relates to a kit for fixing extracellular vesicles in a biological sample.
Claims42
A method of fixing extracellular vesicles, said method comprising:
- providing a sample containing extracellular vesicles and
- contacting the sample with a non-reversible cross-linking agent under conditions effective to fix the extracellular vesicles.
A kit for fixing extracellular vesicles in a biological sample, said kit comprising:
- a support substrate for holding the sample;
- and
- a non-reversible cross-linking agent.
Dependencies are inferred from the claim text.