Device for forming and counting spheroids and method for producing same, and spheroid culturing method and counting method using same

A method for manufacturing a device for forming and counting spheroids, comprising:

• a solution preparation step of preparing a hexanoyl glycol chitosan solution;
• a substrate coating step of coating a substrate by spraying the prepared solution onto the substrate, followed by drying the substrate;
• a mesh member coating step of coating a mesh member by spraying the prepared solution onto the mesh member, followed by drying the mesh member, and
• a step of assembling the coated substrate and the mesh member using an assembly member.

A method for culturing spheroids using a device for forming and counting spheroids comprising:

• seeding a plurality of cells in the device,
• wherein the device comprises a substrate, and a mesh member configured to be positionable on a surface of the substrate and having a plurality of protruding members, wherein the plurality of protruding members intersect one another in the form of a mesh so as to form a plurality of pores;
• and
• obtaining spheroids observed in each pore of the device after several days.

A method for counting spheroids using a device for forming and counting spheroids comprising:

• seeding spheroids in the device,
• wherein the device comprises a substrate, and a mesh member configured to be positionable on a surface of the substrate and having a plurality of protruding members, wherein the plurality of protruding members intersect one another in the form of a mesh so as to form a plurality of pores;
• positioning the spheroids into each pore of the device by shaking the device in both directions parallel to one face of the device;
• placing the device on a stage of a microscope;
• and
• counting the spheroids in a field of view by moving a position on an image in the microscope.