Cell line which is knock out the bmp receptor genes and a method of producing target proteins using the same
AssigneeKorea Advanced Institute of Science and Technology
InventorsGyun-Min LEE, Che Lin KIM
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Abstract
The present invention relates to a transgenic CHO cell line in which the BMP receptor gene BMPRIA or BMPRII is knocked out. The BMP type I receptor BMPRIA or BMP type II receptor BMPRII gene which plays an important role in intracellular signal transduction in CHO cells is knocked out to prevent the activation of self concentration control pathway and the signal transduction mediated by BMP in CHO cells, so that the productivity of a target protein to be produced can be improved.
Claims12
A transgenic CHO cell line wherein a bone morphogenetic protein (BMP) type I receptor gene (BMPRIA) or a BMP type II receptor gene (BMPRII) is knocked out.
A method for preparing a transgenic CHO cell line, wherein the BMP receptor gene BMPRIA or BMPRII is knocked-out, the method comprising the following steps:
- 1) constructing a vector to knock-out the BMP receptor gene BMPRIA or BMPRII;
- 2) introducing the vector of step 1) into a CHO cell line;
- and
- 3) selecting the cell line in which the BMP receptor gene BMPRIA or BMPRII has been knocked-out from those CHO cell lines introduced with the vector of step 2).
Dependencies are inferred from the claim text.