US 2020/0239871 A1 · METHOD, LYSIS SOLUTION AND KIT FOR SELECTIVELY DEPLETING ANIMAL NUCLEIC ACIDS IN A SAMPLE

Abstract

The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

Claims10

Dependent claims

A lysis solution, comprising at least one saponin, a water-soluble salt selected from the group consisting of acetates, sulfates, glutamates, and any mixture thereof;

a viscosity modifying agent selected from the group consisting of sucrose, glucose, and a mixture thereof;
and optionally a polyanionic sulfonate.

Dependencies are inferred from the claim text.

Facts

Application

16/848,117Filed 14 Apr 2020

Priority

20 Oct 2017

Published

30 Jul 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…
C12N 9/22
Acting on ester bonds: ribonucleases [RNase]; Deoxyribonucleases [DNase]
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 2521/301
Phosphoric diester hydrolysing, i.e. nuclease: endonuclease

Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample

Life of this patent

Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample

Life of this patent

Abstract

Claims10