Amplicon Expression Vector Vaccines

A method of prophylactically treating cancer in a subject, said method comprising:

• Without the use of plasmid-derived DNA, assembling an amplicon expression vector template comprising a promotor, an ORF encoding a consensus sequence for telomerase reverse transcriptase and terminator;
• amplifying the amplicon expression vector template via the polymerase chain reaction (PCR) and phosphorothioate modified PCR primers to produce a plurality of amplicon expression vectors;
• purifying and concentrating the amplicon expression vectors to create an efficacious prophylactic dose for the subject;
• and
• administering the prophylactic dose of amplicon expression vectors via electroporation.

A method of treating cancer in a subject, said method comprising:

• Without the use of plasmid-derived DNA, assembling an amplicon expression vector template comprising a promotor, an ORF encoding a consensus sequence for telomerase reverse transcriptase and terminator;
• amplifying the amplicon expression vector template via the polymerase chain reaction (PCR) and phosphorothioate modified PCR primers to produce a plurality of amplicon expression vectors;
• purifying and concentrating the amplicon expression vectors to create an efficacious therapeutic dose for the subject;
• and
• administering the therapeutic dose of amplicon expression vectors via electroporation in conjunction with one or more immune checkpoint inhibitors.