Conversion of methylglyoxal into hydroxyacetone using novel enzymes and applications thereof
AssigneeMetabolic Explorer
InventorsLaetitia FAURE, Gwenaëlle CORRE, Céline RAYNAUD, Laurence DUMON-SEIGNOVERT
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Abstract
The present invention relates to new methylglyoxal reductase (MGR) enzymes which are useful for efficiently converting methylglyoxal into hydroxyacetone. The invention more particularly relates to a method for efficiently converting methylglyoxal into hydroxyacetone using said enzymes, to a method for producing 1,2-propanediol using a microorganism overexpressing said enzymes, and to said microorganism.
Claims21
-15. (canceled)
A method for the fermentative conversion of methylglyoxal into hydroxyacetone, comprising the step of expressing, in a microorganism, at least one methylglyoxal reductase having a catalytic efficiency kcat/Km equal or superior to 5 mM−1s−1 wherein said methylglyoxal reductase is selected from the group consisting of the YjgB enzyme of sequence SEQ ID NO:
- 1 and its mutants.
Dependencies are inferred from the claim text.