High throughput characterization of individual magnetic nanoparticles
AssigneeStc.Unm
InventorsVictor Acosta, Andrejs Jarmola
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Abstract
Technologies related to parallel characterization of individual MNPs are disclosed. A diamond chip with MNPs distributed thereon may be used with an epifluorescence microscope and camera to generate multiple different images of multiple individual MNPs. The multiple images are recorded at different microwave frequencies and under different external magnetic field strengths. The multiple images are then used to determine properties of the multiple individual MNPs.
Claims17
An apparatus for high throughput characterization of individual Magnetic Nanoparticles (MNPs), the apparatus comprising:
- a diamond chip doped with a layer of nitrogen vacancy (NV) color centers;
- one thousand or more MNPs, wherein the MNPs are distributed either on the diamond chip or on a substrate layer on the diamond chip;
- a laser generator to direct a laser beam at the diamond chip;
- a controllable-frequency microwave antenna positioned proximal to the MNPs;
- a controllable-strength magnetic field generator positioned to generate a magnetic field at the MNPs;
- and
- a camera to record fluorescence images, of fluorescence generated by the NV color centers in response to stimulation by the laser beam, wherein the fluorescence recorded in a fluorescence image is modulated by magnetic resonance of the MNPs, and wherein the magnetic resonance is responsive to stimulation by the controllable-frequency microwave antenna under the magnetic field generated by the controllable-strength magnetic field generator.
A method for high throughput characterization of individual Magnetic Nanoparticles (MNPs), comprising:
- distributing one thousand or more MNPs on a diamond chip or on a substrate layer positionable on the diamond chip, wherein the diamond chip is doped with a layer of nitrogen vacancy (NV) color centers;
- positioning the diamond chip and MNPs, or the substrate layer and MNPs, in an epifluorescence microscope, wherein the epifluorescence microscope comprises a camera or is coupled with a camera;
- and
- recording, by the camera, fluorescence images at multiple different microwave frequencies, wherein the fluorescence images record fluorescence modulated by magnetic resonance of the MNPs, wherein each fluorescence image comprises multiple pixels, and wherein at least a first pixel in each fluorescence image records fluorescence modulated by magnetic resonance of a first individual MNP, and wherein at least a second pixel in each fluorescence image records fluorescence modulated by magnetic resonance of a second individual MNP.
Dependencies are inferred from the claim text.