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US 2020/0033357 A1Published application
Viral particle-based protein-protein interaction
AssigneesVib Vzw, Universiteit Gent
InventorsJan Tavernier, Sven Eyckerman
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Abstract
The disclosure relates to a virus-like particle in which a protein complex is entrapped, ensuring the formation of the protein complex under physiological conditions, while protecting the protein complex during purification and identification. The disclosure further relates to the use of such virus-like particle for the isolation and identification of protein complexes.
Claims19
An artificial virus-like particle comprising:
- a viral particle-forming polypeptide;
- a first interaction polypeptide;
- and
- a second interaction polypeptide that interacts with the first interacting polypeptide.
1 more element
A method for detecting a protein-protein interaction, the method comprising:
- expressing a viral particle-forming polypeptide in a cell;
- recruiting a first interaction polypeptide to said viral particle-forming polypeptide;
- recruiting a second interacting polypeptide to the first interaction polypeptide;
- isolating the virus-like particles;
- and
- analyzing the entrapped protein complex so as to detect the protein-protein interaction.
3 more elements
An artificial virus-like particle comprising:
- a viral particle-forming polypeptide, which is a fusion protein comprising HIV p55 GAG protein;
- a first interaction polypeptide that is anchored to the viral particle-forming polypeptide;
- and
- a second interaction polypeptide that interacts with the first interacting polypeptide.
1 more element
Dependencies are inferred from the claim text.