Predicting structured illumination parameters

A non-transitory computer-readable medium having executable instructions stored thereon that, when executed by a processor, cause the processor to perform operations of:

• using a structured illumination system to capture a first image of a sample;
• estimating a first structured illumination parameter using at least the captured first image;
• using the structured illumination system to capture a second image of the sample;
• estimating a second structured illumination parameter using at least the captured second image;
• and
• using at least the first structured illumination parameter or the second structured illumination parameter, predicting a third structured illumination parameter corresponding to a third image.

A structured illumination imaging system, comprising:

• a light emitter to emit light;
• a beam splitter to split light emitted by the light emitter to project a structured illumination pattern on a plane of a sample;
• a processor;
• and
• a non-transitory computer-readable medium having executable instructions stored thereon that, when executed by the processor, cause the processor to perform operations of:
• capturing a first image of a sample;
• estimating a first structured illumination parameter using at least the captured first image;
• capturing a second image of the sample;
• estimating a second structured illumination parameter using at least the captured second image;
• and
• using at least the first structured illumination parameter or the second structured illumination parameter, predicting a third structured illumination parameter corresponding to a third image.

A method, comprising:

• using a structured illumination system to capture a first plurality of images of a sample;
• using a computing device to estimate a first structured illumination parameter using at least the captured first plurality of images;
• using the structured illumination system to capture a second plurality of images of the sample;
• using the computing device to estimate a second structured illumination parameter using at least the captured second plurality of images;
• and
• using at least the first structured illumination parameter or the second structured illumination parameter, using the computing device to predict a third structured illumination parameter corresponding to one or more images.