Light sheet microscope and microscopic method using a light sheet microscope

A microscopic method with a light sheet microscope, the microscopic method comprising:

• providing, by an illumination device, a first illumination beam which is focused to form a first light sheet that illuminates a specimen from a first direction, the first light sheet being inclined obliquely in relation to an optical axis of a specimen-side objective and being guided through the objective;
• and
• detecting, by a detector, detection light passing through the objective,
• wherein the illumination device further provides at least one second illumination beam which is focused to form at least one second light sheet that illuminates the specimen from at least one second direction that differs from the first direction, the at least one second light sheet being inclined obliquely in relation to the optical axis of the objective and being guided through the objective.

A microscopic method with a light sheet microscope, the microscopic method comprising:

• capturing images of a specimen, wherein an angle between image planes and an optical axis of a specimen-side objective of the light sheet microscope is the same and an angle between the image planes is 90°;
• generating image stacks of images of mutually parallel planes;
• and
• merging the image stacks.