Methods, Assays and Kits for Detecting Exposure to Cyanotoxins
AssigneeThe University of Toledo
InventorsDavid Kennedy, Steven Haller, R. Mark Wooten, Deepa Mukundan, Apurva Lad, John Presloid, Jason Huntley, Bruce Levison
Life of this patent
Estimating the term…
Abstract
Methods and kits for the detection of toxic cyanobacteria in a sample by analyzing the sample for the presence antibodies raised in a host, where the presence of antibodies is indicative of toxic cyanobacteria, are described.
Claims16
A method for the detection of toxic cyanobacteria in a sample from a host, comprising:
- analyzing the sample for the presence antibodies raised in the host, wherein the presence of the antibodies is indicative of toxic cyanobacteria.
A method for the detection of toxic cyanobacteria in an assay, comprising:
- analyzing the sample for the presence antibodies complexed to an immobilized microcystin, wherein the presence of the antibodies is indicative of toxic cyanobacteria.
A method for the detection of microcystins in a sample, comprising:
- a) providing a substrate coated with a protein capable of conjugating with the ADDA region of microcystin;
- b) adding dilutions of the sample to the coated substrate;
- c) washing excess sample from the coated substrate,
- d) detecting any bound anti-ADDA antibodies from the sample using a labeled antibody;
- and,
- e) adding an enzymatic substrate to produce a visible signal, which indicates the presence of microcystins in the sample.
A kit for the detection of toxic cyanobacteria in a sample, the kit comprising at least one a protein capable of conjugating with the ADDA region of microcystin designed to detect toxin-associated antibodies in a sample.
An assay for detecting the presence or quantity of bacteria within a sample, the assay comprising:
- an assay device;
- and
- a substrate inserted into the assay device;
- the substrate having capture antibodies and detection antibodies,
- the detection antibodies comprising a protein capable of conjugating with the ADDA region of microcystins;
- the capture antibodies comprising an enzymatic substrate capable of producing a visible signal.
Dependencies are inferred from the claim text.