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US 2020/0003774 A1Published application
Biosensor for detecting smell, scent, and taste
AssigneeAromyx Corporation
InventorsChris HANSON, William HARRIES, Victor Todd CUSHMAN, Ed COSTELLO
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Abstract
The invention relates to biosensors for detecting odorants, especially a biosensor that mimics odorant detection by a mammal, for example, humans, dogs or cats. The field of the invention also related to the standardization of odors for scent, smell and taste using the biosensor of the invention, and the discovery of agonists, antagonists, and mixtures of odorants for creating new odors, masking odors, enhancing odors, and designing odors.
Claims41
1-20. (canceled)
21:
- A system comprising:
- a plurality of biosensors, each biosensor comprising a heterologous olfactory receptor in a membrane fraction of a host cell;
- a reporter coupled to the heterologous olfactory receptor, wherein the reporter produces a signal upon binding of the heterologous olfactory receptor to an odorant;
- and
- a detector that measures the signal produced by the reporter.
2 more elements
22:
- The system of claim 21, wherein the host cell is a yeast cell.
23:
- The system of claim 21, wherein the host cell is a mammalian cell.
24:
- The system of claim 21, wherein the heterologous human olfactory receptor is at least one from the OR1 family, OR2 family, OR3 family, OR 4 family, OR5 family, OR6 family, OR7 family, OR8 family, OR9 family, OR10 family, OR11 family, OR12 family, OR13 family, OR14 family, OR51 family, OR52 family, OR55 family, or OR56 family.
25:
- The system of claim 24, wherein the heterologous human olfactory receptor is at least one from the OR1 family.
26:
- The system of claim 25, wherein the at least one heterologous human olfactory receptor from the OR1 family is OR1A1.
27:
- The system of claim 21, wherein the plurality of biosensors comprise the same heterologous olfactory receptor.
28:
- The system of claim 21, wherein the plurality of biosensors comprise different heterologous olfactory receptors.
29:
- The system of claim 28, wherein the different heterologous olfactory receptors are from different olfactory receptor families.
30:
- The system of claim 21, wherein the odorant is associated with a characteristic smell, odor, or aroma of a composition.
31:
- The system of claim 21, wherein the odorant is associated with a characteristic taste of a composition.
32:
- The system of claim 21, wherein the odorant is in the gaseous phase.
33:
- The system of claim 21, wherein the odorant is in solution.
34:
- A method of identifying one or more odorants in a composition comprising adding the composition to the system of claim 21, and identifying the one or more odorants by detecting the signal produced by the reporter.
35:
- The method of claim 34, wherein signal is detected from the one or more odorants in the gaseous phase.
36:
- The method of claim 34, wherein the signal is detected from the one or more odorants in solution.
37:
- The method of claim 34, wherein the heterologous human olfactory receptor is at least one from the OR1 family, OR2 family, OR3 family, OR 4 family, OR5 family, OR6 family, OR7 family, OR8 family, OR9 family, OR10 family, OR11 family, OR12 family, OR13 family, OR14 family, OR51 family, OR52 family, OR55 family, or OR56 family.
38:
- The method of claim 37, wherein the heterologous human olfactory receptor is at least one from the OR1 family.
39:
- The method of claim 38, wherein the at least one heterologous human olfactory receptor from the OR1 family is OR1A1.
A system comprising:
- a plurality of biosensors, each biosensor comprising a heterologous human olfactory receptor from the OR1 family, OR2 family, OR3 family, OR 4 family, OR5 family, OR6 family, OR7 family, OR8 family, OR9 family, OR10 family, OR11 family, OR12 family, OR13 family, OR14 family, OR51 family, OR52 family, OR55 family, OR56 family, or a combination thereof, expressed in a host cell, the host cell further comprising a reporter coupled to the heterologous human olfactory receptor, and human G protein subunits Gα, Gβ, and Gγ, wherein the reporter produces a signal upon binding of the heterologous human olfactory receptor to an odorant;
- and
- a detector that measures the signal produced by the reporter.
41:
- The system of claim 40, wherein the plurality of biosensors comprise the same heterologous olfactory receptor.
42:
- The system of claim 40, wherein the plurality of biosensors comprise different heterologous olfactory receptors.
43:
- The system of claim 40, wherein the odorant is associated with a characteristic smell, odor, or aroma of a composition.
44:
- The system of claim 40, wherein the odorant is associated with a characteristic taste of a composition.
45:
- The system of claim 40, wherein the odorant is in the gaseous phase.
46:
- The system of claim 40, wherein the odorant is in solution.
47:
- The system of claim 40, wherein the heterologous human olfactory receptor is from the OR1 family.
48:
- The system of claim 47, wherein the heterologous human olfactory receptor from the OR1 family is OR1A1.
49:
- The system of claim 40, wherein the host cell is a yeast cell.
50:
- The system of claim 40, wherein the host cell is a mammalian cell.
51:
- The system of claim 40, wherein the reporter is a fluorescent reporter, a bioluminescent reporter, an enzyme, or an ion channel.
52:
- A method for identifying one or more odorants in a composition comprising:
- exposing a plurality of biosensors to the composition, each biosensor comprising an olfactory receptor expressed in a host cell, the host cell further expressing a reporter coupled to the olfactory receptor, wherein the reporter produces a signal upon binding of the olfactory receptor to the one or more odorants;
- and
- identifying the one or more odorants by detecting the signal produced by the reporter,
- wherein the olfactory receptor is a heterologous human olfactory receptor from the OR1 family, OR2 family, OR3 family, OR 4 family, OR5 family, OR6 family, OR7 family, OR8 family, OR9 family, OR10 family, OR11 family, OR12 family, OR13 family, OR14 family, OR51 family, OR52 family, OR55 family, OR56 family, or a combination thereof, and
- wherein the odorant is associated with a characteristic scent, odor, aroma, or taste of the composition.
3 more elements
53:
- The method of claim 52, wherein the host cell further expresses human G protein subunits Gα, Gβ, and Gγ.
54:
- The method of claim 52, further comprising generating an aromagraph from the detected signals produced by the reporters.
55:
- The method of claim 54, wherein the composition is recreated according to the aromagraph.
56:
- The method of claim 54, wherein the aromagraph is used to remediate a malodor.
57:
- The method of claim 52, wherein the host cell is a yeast cell.
58:
- The method of claim 52, wherein the host cell is a mammalian cell.
59:
- The method of claim 52, wherein signal is detected from the one or more odorants in the gaseous phase.
60:
- The method of claim 52, wherein the signal is detected from the one or more odorants in solution.
Dependencies are inferred from the claim text.