Fusion proteins containing luciferase and a polypeptide of interest
AssigneeThe Government of the United States of America, as represented by the Secretary of Homeland Security
InventorsMichael Puckette, Max V. Rasmussen
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Abstract
Polynucleotides encoding fusion proteins contain a secretable luciferase fused to a modified polypeptide of interest are disclosed. The polypeptide of interest has been modified to remove a native N-terminal secretion sequence and has been replaced by the secretable luciferase. One example of a modified polypeptide of interest is interferon. The polynucleotides and fusion proteins have biotherapeutic, diagnostic, and quality control applications in biotechnological, medical, and veterinary fields. Methods for producing the secretable fusion protein are also disclosed.
Claims16
A polynucleotide that encodes a fusion protein comprising a luciferase, an interferon, and FMDV Δ1D2A.
A polynucleotide that encodes a fusion protein comprising, in order from the N-terminal, a secretable luciferase fused to a modified polypeptide of interest, wherein:
- the modified polypeptide of interest has been modified to remove a native N-terminal secretion sequence;
- the removed N-terminal secretion sequence has been replaced by the secretable luciferase;
- and
- the polynucleotides encoding the secretable luciferase are directly adjoined to the polynucleotides encoding the polypeptide of interest.
A fusion protein comprising, in order from the N-terminal, a secretable luciferase and a modified polypeptide of interest, wherein:
- the modified polypeptide of interest has been modified to remove a native N-terminal secretion sequence;
- the N-terminal secretion domain has been replaced by the luciferase;
- and
- the luciferase is directly adjoined to the modified polypeptide of interest.
A method for producing a secretable fusion protein, comprising:
- providing a host cell expressing a polynucleotide encoding the fusion protein;
- culturing the host cell in a suitable medium, wherein the secretable fusion protein is expressed and secreted by the host cell into the medium;
- and
- recovering the secreted fusion protein from the medium,
- wherein:
- the fusion protein comprises, in order from the N-terminal, a secretable luciferase and a modified polypeptide of interest;
- the modified polypeptide of interest has been modified to remove a native N-terminal secretion domain;
- the native N-terminal secretion domain has been replaced by the secretable luciferase;
- and
- the luciferase is directly adjoined to the polypeptide of interest.
Dependencies are inferred from the claim text.