Lipases with increased thermostability
AssigneeHenkel AG & Co. KGaA
InventorsNINA MUSSMANN, SUSANNE WIELAND, DANIELA HERBST, MARGRET VAN LIER, ROLAND WEIS
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AssigneeHenkel AG & Co. KGaA
InventorsNINA MUSSMANN, SUSANNE WIELAND, DANIELA HERBST, MARGRET VAN LIER, ROLAND WEIS
Estimating the term…
The Present disclosure relates to lipases comprising an amino acid sequence which has at least 70% sequence identity with the amino acid sequence specified in SEQ ID NO:1 over the entire length thereof and which have an amino acid substitution in at least one of the positions S93, P145, L156, K169, N193, G202, Q225, K235, V236, P260, H298, P308, F311, N328, L352, D359 or S364, referring in each case to the numbering according to SEQ ID NO:1, and the production and use thereof. Lipases of such kind have very good stability, particularly thermal stability, and at the same time good cleaning power.
Lipase comprising an amino acid sequence which has the at least about 70% sequence identity with the amino acid sequence specified in SEQ ID NO:1 over the entire length thereof and which has an amino acid substitution in at least one of the position S93, P145, L156, K169, N193, G202, Q225, K235, V236, P260, H298, P308, F311, N328, L352, D359 or S364, referring in each case to the numbering according to SEQ ID NO:1.
Method for producing a lipase comprising substituting an amino acid in at least one of the positions corresponding to positions 93, 145, 156, 169, 193, 202, 225, 235, 236, 260, 298, 308, 311, 328, 352, 359 and 364 in SEQ ID NO:1 in a starter lipase which has at least about 70% sequence identity with the amino acid sequence specified in SEQ ID NO:1 over the entire length thereof.
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Dependencies are inferred from the claim text.