Polymerase enzyme from 9°n

A polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70% amino acid sequence identity thereto, comprising the following mutation(s):

• i. at position 408 of SEQ ID NO. 1 a mutation selected from:
• 1. (H—histidine) (L408H),
• 2. (M—methionine) (L408M),
• 3. (T—threonine) (L408T),
• 4. (G—glycine) (L408M),
• 5. (N—asparagine) (L408N), and
• 6. (S—serine) (L408S)
• ii. at position 409 of SEQ ID NO. 1 a mutation selected from:
• 1. (T—threonine) (Y409T),
• 2. (V—valine) (Y409V), and
• 3. (G—glycine) (Y409G)
• iii. at position 410 of SEQ ID NO. 1 a mutation selected from:
• 1. (C—cysteine) (P410C),
• 2. (N—asparagine) (P410N),
• 3. (D—aspartic acid) (P410D),
• 4. (Q—glutamine) (P410Q),
• 5. (F—phenylalanine) (P410F), and
• 6. (S—serine) (P410S)
• wherein the enzyme has little or no 3′-5′ exonuclease activity.