Back to results
US 2020/0002672 A1Published application
Activation of immune cells
AssigneeBreakthrough Tech LLC
InventorFrancis Christopher Zeigler
Life of this patent
Estimating the term…
Abstract
Bone marrow containers are described herein. Separation systems for separating activated immune cells from other components are described herein. Methods of activating an immune cell are described herein.
Claims11
-10. (canceled)
A method of activating immune cells of a subject, the method comprising:
- obtaining bone marrow of the subject, the bone marrow comprising immune cells;
- incubating the bone marrow with a non-ionic hydrophilic branched polysaccharide and a TLR2 ligand, wherein the non-ionic hydrophilic branched polysaccharide comprises poly(sucrose-co-epichlorhydrin),
- wherein the non-ionic hydrophilic branched polysaccharide is at a concentration, wherein, if the bone marrow is contacted with whole blood and centrifuged, said concentration is sufficient to permit fluid movement of whole blood through the non-ionic hydrophilic branched polysaccharide without separation of erythrocytes,
- wherein the non-ionic hydrophilic branched polysaccharide has a molecular weight greater than 20 kDa, and
- wherein the incubating is performed until the immune cells are activated;
- and
- administering the activated immune cells to the subject.
4 more elements
(canceled)
Dependencies are inferred from the claim text.