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AssigneeThe University Court of the University of Aberdeen
InventorsCaroline Jane BARELLE, William James Jonathan FINLAY, Alfredo DARMAN-IN-SHEEMAN, Andrew Justin Radcliffe PORTER
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The present invention provides methods for the production of a library of antigen specific antigen binding molecules having a peptide domain structure represented by the following formula (I): FW 1-CDR1-FW2-HV2-FW3a-HV4-FW3b-CDR3-FW4 comprising (1) isolating RNA from a member of a species in the Elasmobranchii subclass; (2) amplifying DNA sequences from RNA obtained; (3) selecting a DNA sequence from the database prepared; (4) amplifying DNA sequences encoding two or more contiguous peptide domains of FW1-CDR1-FW2-HV2-FW3a-HV4-FW3b-CDR3-FW4; (5) ligating together said amplified DNA sequences to form DNA sequences encoding an antigen specific binding molecule; (6) cloning the amplified DNA obtained into a display vector; and (7) transforming a host with said display vector to produce a library of said antigen specific antigen binding molecules. The invention also provides methods for the production of an antigen specific antigen binding molecule as defined, pharmaceutical compositions comprising such molecules and uses thereof in medicine.
1-25. (canceled)
A process for the production of an antigen specific antigen binding molecule using a transformed host containing a library of expressible DNA sequences encoding a plurality of antigen specific antigen binding molecules wherein the library is created from at least two heterologous isotype NAR sequences, wherein the antigen specific antigen binding molecules comprise a plurality of domains of a variable region of the immunoglobulin isotype NAR found in a member of a species in the Elasmobranchii subclass.
Dependencies are inferred from the claim text.