Fluorescent methods and materials for directed biomarker signal amplification

A multichromophore-biomolecule conjugate comprising:

• a sensor biomolecule covalently linked to a light harvesting water soluble multichromophore via a bioconjugation site;
• wherein the light harvesting water soluble multichromophore is a conjugated polymer having a backbone of π-conjugated repeat units and comprises the structure:
• {{[CP1]-a[CP2]b}m[CP1]-a[CP3]c}n
• wherein:
• CP1 is present and is a polycyclic repeat unit;
• CP2 is an aromatic repeat unit;
• CP3 is an aromatic repeat unit comprising a bioconjugation functional group capable of covalently linking to a biomolecule or dye;
• m is 1 to 10,0000;
• n is independently 0 to 10,000 and;
• and
• a, b and c are independently 0 to 250 wherein a+b+c>1.

A light harvesting water soluble multichromophore comprising a conjugated polymer having a backbone of π-conjugated repeat units and a bioconjugation functional group capable of covalently linking to a sensor biomolecule, wherein the multichromophore has the structure:

• {{[CP1]-a[CP2]b}m[CP1]-a[CP3]c}n
• wherein:
• CP1 is present and is a polycyclic repeat unit;
• CP2 is an aromatic repeat unit;
• CP3 is an aromatic repeat unit comprising a bioconjugation functional group capable of covalently linking to a biomolecule or dye;
• m is 1 to 10,0000;
• n is independently 0 to 10,000 and;
• and
• a, b and c are independently 0 to 250 wherein a+b+c>1.