Method and Device for Combined Detection of Viral and Bacterial Infections

A method to simultaneously detect at least one extracellular analyte and at least one intracellular analyte, comprising the steps of:

• a) collecting a sample;
• b) transferring the sample to a sample analysis device;
• c) lysing the sample;
• and
• d) simultaneously detecting the extracellular analyte and the intracellular analyte on the sample analysis device.

A method of detecting MxA protein and C-reactive protein in a sample, comprising the steps of:

• a) adding the sample to a mixture of an antibody to MxA protein conjugated to a first label and an antibody to C-reactive protein conjugated to a second label different from the first label;
• b) detecting a presence of MxA protein by determining whether the antibody to MxA protein has agglutinated;
• and
• c) detecting a presence of C-reactive protein by determining whether the antibody to C-reactive protein has agglutinated.

A method of detecting the presence of an unknown viral infection, comprising the steps of:

• a) collecting a sample;
• b) transferring the sample to a sample application zone of a sample analysis device comprising:
• i) a conjugate zone comprising a sialic acid nanomicelle comprising a label inside the nanomicelle;
• and
• ii) a detection zone laterally downstream from the sample application zone, comprising a sialic acid homolog nanoparticle;
• c) analyzing the sample for a positive result in the detection zone.

A method of detecting the presence of a specific viral infection, comprising the steps of:

• a) collecting a sample;
• b) transferring the sample to a sample application zone of a sample analysis device comprising:
• i) a conjugate zone comprising a molecule selected from the group consisting of:
• a nanomicelle comprising a binding partner for a specific virus that causes the viral infection and a label;
• and a sialic acid homolog nanomicelle comprising a label inside the nanomicelle;
• and
• ii) a detection zone laterally downstream from the sample application zone, comprising a nanoparticle specific for the virus that causes the viral infection.
• c) analyzing the sample for a positive result in the detection zone.