Genetically encoded biosensors
AssigneeHoward Hughes Medical Institute
InventorsJonathan S. Marvin, Loren Looger
Life of this patent
Estimating the term…
Abstract
The present disclosure provides, inter alia, genetically encoded recombinant peptide biosensors comprising analyte-binding framework portions and signaling portions, wherein the signaling portions are present within the framework portions at sites or amino acid positions that undergo a conformational change upon interaction of the framework portion with an analyte.
Claims44
A recombinant peptide biosensor comprising an analyte-binding framework portion and a signaling portion, wherein the signaling portion is present within the framework portion at a site or amino acid position that undergoes a conformational change upon interaction of the framework portion with a defined, specific, or selected analyte.
A recombinant peptide biosensor comprising an amino acid sequence with at least 90% identity to a recombinant peptide biosensor selected from the group consisting of SEQ ID NOs:
- 176-182, wherein the recombinant peptide biosensor binds specifically to glutamate.
A recombinant peptide biosensor comprising an amino acid sequence with at least 90% identity to a recombinant peptide biosensor having the sequence shown in SEQ ID NO:
- 183, wherein the recombinant peptide biosensor binds specifically to glucose.
A recombinant peptide biosensor comprising an amino acid sequence with at least 90% identity to a recombinant peptide biosensor having the sequence shown in SEQ ID NO:
- 184, wherein the recombinant peptide biosensor binds specifically to GABA.
A recombinant peptide biosensor comprising an amino acid sequence with at least 90% identity to a recombinant peptide biosensor having a sequence selected from the group consisting of SEQ ID NO:
- 185 and 186, wherein the recombinant peptide biosensor binds specifically to ACh.
A method for detecting glutamate, the method comprising detecting a level of fluorescence emitted by a recombinant peptide biosensor, the peptide biosensor having an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 176-182, and correlating the level of fluorescence with the presence of glutamate.
A method for detecting glucose, the method comprising detecting a level of fluorescence emitted by a recombinant peptide biosensor, the peptide biosensor having an amino acid sequence shown in SEQ ID NO:
- 183, and correlating the level of fluorescence with the presence of glucose.
A method for detecting GABA, the method comprising detecting a level of fluorescence emitted by a recombinant peptide biosensor, the peptide biosensor having an amino acid sequence shown in SEQ ID NO:
- 184, and correlating the level of fluorescence with the presence of GABA.
A method for detecting ACh, the method comprising detecting a level of fluorescence emitted by a recombinant peptide biosensor, the peptide biosensor having an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 185 and 186, and correlating the level of fluorescence with the presence of ACh.
Dependencies are inferred from the claim text.