Solid phase conjugate
AssigneeBBI Solutions OEM Limited
InventorsFfion Walters, Morgan West
Life of this patent
Estimating the term…
Abstract
The present invention relates to a solid phase biomolecule conjugate for use in a detection assay, comprising on the surface thereof a population of oligonucleotides, and a specific binding partner. The oligonucleotides serve to block the sites which are not bound by a specific binding partner and increase sensitivity for a target molecule to be detected in a sample.
Claims31
A solid phase biomolecule conjugate for specific binding to a target molecule, the conjugate comprising a solid phase comprising a surface, wherein the surface has bound thereto i) a binding partner specific for the target molecule, and ii) a population of oligonucleotides which do not bind to the target molecule, wherein each oligonucleotide is between 2 to 200 nucleotides in length.
4-5. (canceled)
10-12. (canceled)
15-21. (canceled)
23-28. (canceled)
31-32. (canceled)
A method of detecting a target molecule, the method comprising
- a) providing a solid-phase biomolecule conjugate, wherein the conjugate comprises a solid phase having a surface, wherein the surface has attached thereto i) a specific binding partner for the target molecule, and ii) a population of oligonucleotides which do not bind the target molecule, and wherein each oligonucleotide is between 2 to 200 nucleotides in length;
- b) contacting the solid-phase biomolecule conjugate with a sample suspected of containing the target molecule;
- c) incubating the sample and the conjugate for sufficient time to allow specific binding of the conjugate to any target molecule present in the sample;
- and
- d) detecting a change which occurs upon binding of any target molecule to the conjugate.
(canceled)
(canceled)
(canceled)
(canceled)
43-46. (canceled)
49-51. (canceled)
Dependencies are inferred from the claim text.