Anastasis biosensor

6-9. (canceled)

A biosensor system comprising:

• a. a site-specific recombinase tethered to the plasma membrane of a test cell, wherein the recombinase is linked to a transmembrane domain via an enzyme cleavable linker;
• and
• b. a nucleic acid encoding a reporter gene operably linked to a promoter, wherein the recognition target sequence of the recombinase flanks a stop codon cassette located between the reporter gene and the promoter.

A biosensor system comprising:

• a. a site-specific recombinase tethered to the plasma membrane of a test cell, wherein the recombinase is linked to a transmembrane domain via an enzyme cleavable linker;
• and
• b. a nucleic acid encoding a reporter gene operably linked to a promoter, wherein the recognition target sequence of the recombinase flanks a stop codon cassette located between the reporter gene and the promoter.

16-19. (canceled)

A biosensor comprising a nucleic acid comprising a stop codon cassette flanked by site specific recombinase recombination sequences, wherein the stop codon cassette and flanking sequences separate a constitutive promoter and a fluorescent protein open reading frame.