Method for cancer grading

A method for establishing an index of a given cancer grade, comprising:

• providing a reference cancer cell sample, wherein the reference cancer cell sample has the given cancer grade and comprises glycan chains anchored on the surface of the reference cancer cell sample;
• providing a first adsorbent and a second adsorbent, wherein the carbon number of the first adsorbent and the second adsorbent is from to 46, and the carbon number of the first adsorbent is less than that of the second adsorbent;
• profiling glycan distribution pattern of the glycan chains anchored on the surface of the reference cancer cell sample;
• adsorbing the profiled reference cancer cell sample with the first adsorbent and the second adsorbent, respectively;
• measuring the amount of the first adsorbent adhering onto the profiled reference cancer cell sample and measuring the amount of the second adsorbent adhering onto the profiled reference cancer cell sample, respectively;
• and
• acquiring a first reference correlation between the glycan distribution pattern of the reference cancer cell sample and the amount of the first adsorbent adhering onto the profiled reference cancer cell sample;
• acquiring a second reference correlation between the glycan distribution pattern of the reference cancer cell sample and the amount of the second adsorbent adhering onto the profiled reference cancer cell sample, wherein the first reference correlation and the second reference correlation form the index of the given cancer grade.