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US 2019/0376934 A1Published application
Sample preparation for proteomic investigations
AssigneeFederal Republic of Germany Last Represented By the Robert-Koch-Institut Which Is Represneted By
InventorsAndy SCHNEIDER, Jörg DÖLLINGER, Peter LASCH
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Abstract
A method for disrupting a sample of biological material for proteome analysis by a mass-spectrometry method is provided, involving admixing the sample with a certain volume of an organic acid, incubating said sample for a certain period for lysis and then neutralizing said sample.
Claims12
A method for disrupting a sample of biological material for proteome analysis by a mass-spectrometry method, comprising the steps of:
- providing the sample in a reaction vessel,
- adding a certain amount of an organic acid to the sample,
- incubating the preparation,
- adding a neutralizing solution to the preparation.
1 more element
(canceled)
(canceled)
A method for lysing a biological material, comprising:
- adding trifluoroacetic acid to the biological material;
- and
- lysing the biological material.
Dependencies are inferred from the claim text.