Size exclusion chromatography of biological molecules
AssigneeWaters Technologies Corporation
InventorsKevin Wyndham, Matthew A. Lauber
Life of this patent
Estimating the term…
Abstract
The present invention is directed to a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for improving the speed and separations of size exclusion chromatography using a stationary phase material comprising small particles (<2 micron in diameter).
Claims31
A method of performing size exclusion chromatography comprising the steps of
- a) providing a housing having at least one wall defining a chamber having an entrance and an exit;
- and a stationary phase material comprising a core and surface composition held in said chamber;
- wherein said stationary phase material comprises particles having diameters with a mean size distribution of less than 2.0 microns;
- b) loading a sample on said stationary phase material in said chamber at a column inlet pressure of greater than 500 psi and flowing the sample through said stationary phase material;
- and
- c) separating the sample into one or more biomolecule analytes by size.
A method of performing size exclusion chromatography comprising the steps of
- a) providing a housing having at least one wall defining a chamber having an entrance and an exit;
- wherein the housing comprises a wide bore column of a bore size of 7.8 mm i.d or more;
- and a stationary phase material comprising a core and surface composition held in said chamber;
- wherein said stationary phase material comprises particles having diameters with a mean size distribution of less than 2.0 microns;
- b) loading a sample on said stationary phase material in said chamber at a column inlet pressure of greater than 500 psi and flowing the sample through said stationary phase material;
- and
- c) separating the sample into one or more biomolecule analytes by size.
A method of performing size exclusion chromatography comprising the steps of
- a) providing a housing having at least one wall defining a chamber having an entrance and an exit;
- wherein the length of the chamber is about 50 mm;
- and wherein the housing comprises a wide bore column of a bore size of 7.8 mm i.d or more;
- and a stationary phase material comprising a core and surface composition held in said chamber;
- wherein said stationary phase material comprises particles having diameters with a mean size distribution of less than 2.0 microns;
- b) loading a sample on said stationary phase material in said chamber at a column inlet pressure of greater than 500 psi and flowing the sample through said stationary phase material;
- and
- c) separating the sample into one or more biomolecule analytes by size.
(canceled)
(canceled)
8-11. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
21-23. (canceled)
25-28. (canceled)
(canceled)
33-38. (canceled)
Dependencies are inferred from the claim text.