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US 2019/0376902 A1Published application
Optical imaging system using lateral illumination for digital assays
AssigneeAbbott Japan Co., Ltd.
InventorTomohiro Ikeda
Life of this patent
Estimating the term…
Abstract
A compact optical imaging system including a single filter and a light source that provides lateral illumination for bead detection in digital assays. The light source is configured to emit light toward the detection vessel. The single filter is positioned to receive light reflected from a sample in the detection vessel, that originated from the light source, and receive an output from a sample in the detection vessel. A detector is configured to receive a portion of the reflected light and a portion of the output that passes through the single filter.
Claims54
-53. (canceled)
A method of detecting an analyte of interest in a liquid droplet, which method comprises:
- (a) providing a first liquid droplet containing an analyte of interest and a second liquid droplet containing at least one solid support, wherein the solid support comprises a specific binding member that binds to the analyte of interest;
- (b) using energy to exert a force to manipulate the first liquid droplet with the second liquid droplet to create a mixture;
- (c) moving all or at least a portion of the mixture to an array of wells;
- (d) adding at least one detectable label to the mixture before and/or after step (c);
- and
- (e) detecting the analyte of interest in the wells using a compact digital assay apparatus comprising:
- (i) a detection vessel;
- (ii) a light source configured to emit light toward the detection vessel;
- (iii) a single filter positioned to receive and pass through a portion of light reflected from a sample in the detection vessel, that originated from the light source, and receive and pass through a portion of a fluorescence output from a sample in the detection vessel;
- and
- (iv) a detector configured to receive a portion of the reflected light and a portion of the fluorescence output that passes through the single filter.
8 more elements
A method of detecting an analyte of interest in a liquid droplet, which method comprises:
- (a) providing a first liquid droplet containing an analyte of interest and a second liquid droplet containing at least one solid support, wherein the solid support comprises a specific binding member that binds to the analyte of interest;
- (b) using energy to exert a force to manipulate the first liquid droplet with the second liquid droplet to create a mixture;
- (c) moving all or at least a portion of the mixture to an array of wells;
- (d) adding at least one detectable label to the mixture before and/or after step (c);
- and
- (e) detecting the analyte of interest in the wells using a compact digital assay apparatus comprising:
- (i) a detection vessel;
- (ii) a first light source configured to emit light toward the detection vessel and at an angle relative to the detection vessel;
- (iii) a second light source configured to emit light toward the detection vessel;
- (iv) a single filter positioned to receive and pass through a portion of light reflected from a sample in the detection vessel, that originated from the first light source, and receive and pass through a portion of a fluorescence output from a sample in the detection vessel;
- and
- (v) a detector configured to receive a portion of the reflected light and a portion of the fluorescence that passes through the single filter.
9 more elements
A method of detecting an analyte of interest in a liquid droplet, which method comprises:
- (a) providing a first liquid droplet containing an analyte of interest and a second liquid droplet containing at least one solid support, wherein the solid support comprises a specific binding member that binds to the analyte of interest;
- (b) using energy to exert a force to manipulate the first liquid droplet with the second liquid droplet to create a mixture;
- (c) moving all or at least a portion of the mixture to an array of wells;
- (d) adding at least one detectable label to the mixture before and/or after step (c);
- and
- (e) detecting the analyte of interest in the wells using a compact digital assay apparatus comprising:
- (i) a sample array having a plurality of detection vessels;
- (ii) a first light source configured to emit light toward the detection vessels at an angle relative to the detection vessels to illuminate a sample in the detection vessels;
- (iii) a second light source configured to emit light toward the detection vessels without using a mirror or other reflective object, the second light source further configured to activate a sample in the detection vessels to emit a fluorescence output;
- (iv) a filter positioned to receive and pass through a portion of light reflected from the detection vessels that originated from the first light source, and receive and pass through a portion of the fluorescence output from the sample in the detection vessels;
- and
- (v) a detector configured to receive the reflected light and the fluorescence output from the sample that pass through the filter and to generate optical data identifying which vessels contain a bead and which vessels contain a label.
9 more elements
A method of detecting an analyte of interest in a liquid droplet, which method comprises:
- (a) providing a first liquid droplet containing an analyte of interest and a second liquid droplet containing at least one solid support, wherein the solid support comprises a specific binding member that binds to the analyte of interest;
- (b) using energy to exert a force to manipulate the first liquid droplet with the second liquid droplet to create a mixture;
- (c) moving all or at least a portion of the mixture to an array of wells;
- (d) adding at least one detectable label to the mixture before and/or after step (c);
- and
- (e) detecting the analyte of interest using a compact digital assay apparatus comprising:
- (i) a sample array including a plurality of samples positioned in a plurality of nanowells;
- (ii) a light source configured to emit light toward the sample array at an angle relative to the sample array to illuminate a sample in the sample array, the light source having a wavelength between 450 nm and 550 nm;
- (iii) a single filter positioned to receive and pass through a portion of light reflected from the plurality of samples in the sample array, that originated from the light source;
- and
- (iv) a detector configured to receive a portion of light reflected from the sample.
8 more elements
Dependencies are inferred from the claim text.