Hardware And Mobile Software For Operation Of Portable Instruments For Nucleic Acid Amplification

A method of detecting amplified nucleic acid molecules, the method comprising introducing sample into a device configured for detection of amplified nucleic acid molecules and detecting the amplified nucleic acid molecules, wherein the device comprises:

• a first housing unit and a second housing unit,
• wherein the first and second housing units are connectable to each other by a fastener,
• wherein the interface between the first and second housing units comprises a gasket;
• wherein the connected first and second housing units enclose the following:
• a) a reaction block;
• b) a circuit board electronically connected to the reaction block;
• and
• c) a battery electronically connected to the circuit board;
• wherein the first housing unit comprises an access area for communication with the reaction block,
• wherein the reaction block comprises:
• a first bank of light source and a second bank of light source on opposite sides of a heated receptacle,
• wherein the heated receptacle comprises a plurality of transverse orifices, and wherein the heated receptacle comprises a plurality of bottom portals;
• a first and second excitation filter in light communication between the first bank of light source and the plurality of transverse orifices of the heated receptacle and second bank of light source and the plurality of transverse orifices of the heated receptacle, respectively;
• a first detector bank and a second detector bank in light communication with the plurality of bottom portals of the heated receptacle;
• and a first emission filter and a second emission filter in light communication between the plurality of bottom portals of the heated receptacle and the first detector bank and a second detector bank, respectively;
• wherein the first housing unit comprises a reaction lid closing off the access with the reaction block.