Systems, devices and methods for cell capture and methods of manufacture thereof

A flowchip configured for manufacturing a capture substrate, comprising:

• a substrate comprising a compressible material;
• a plurality of inlets;
• a plurality of outlets;
• a plurality of flowchannels,
• wherein:
• the substrate is configured for reversible sealing engagement against a capture substrate.

A method for manufacturing a capture substrate, comprising:

• providing a flowchip comprising a compressible substrate, the substrate including a plurality flowchannels, each flow channel including a corresponding inlet and outlet;
• arranging a substrate to cover and removably attached to at least the plurality of flow channels of the compressible substrate, the substrate being removably attached to the compressible substrates via negative pressure;
• providing a sample adjacent each inlet;
• and
• applying a negative pressure to each of the plurality of inlets such that the sample is distributed within each flow channel such that each sample is deposited on a portion of the substrate which corresponds to a respective flow channel,
• wherein after a predetermined period of time of applied pressure, capture areas (CAs) are formed on each portion.