Composition and Method for Disrupting Tissue Material
OwnerDominic O'NEIL, Tanya SPERLING +1inventors
InventorsDominic O'NEIL, Tanya SPERLING, Stefanie SCHROEER
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Abstract
The present invention relates to a composition for disrupting tissue material, the composition comprising solid disrupting particles in combination with at least one enzyme for enzymatic lysis and at least one chaotropic agent , as well as to a method for disrupting tissues material by simultaneously applying mechanical grinding or milling disruption and enzymatic digestion.
Claims31
A composition for disrupting solid tissue material, comprising
- one or more solid disrupting particles;
- at least one enzyme for enzymatic lysis;
- at least one chaotropic agent in a total concentration equal to or less than 1M;
- and which does not comprise an alcohol in an amount, which denatures or inactivates the at least one enzyme.
A kit for disrupting tissue material, the kit comprising
- one or more solid disrupting particles;
- at least one enzyme for enzymatic lysis;
- at least one buffer;
- at least one chaotropic agent in a total concentration equal to or less than 1 M;
- and optionally one or more agents selected from
- at least one surfactant;
- at least one nuclease inhibitor;
- at least one anti-foaming agent;
- at least one osmotic stabilizer;
- at least one reducing agent;
- at least one additional enzyme, other than the enzyme for enzymatic lysis;
- at least one organic solvent;
- and
- optionally a container for receiving the tissue material;
- and a leaflet with instructions for processing the tissue material.
A method for disrupting tissue material, wherein the tissue material is subjected to a simultaneous treatment of
- mechanical disruption by grinding, milling or beating and
- chemical disruption by enzymatic lysis,
- by grinding, milling or beating the tissue material in a lysis solution which comprises one or more solid disrupting particles, at least one enzyme for enzymatic lysis, and at least one chaotropic agent in a total concentration equal to or less than 1M and which does not comprise an alcohol in an amount, which denatures or inactivates the at least one enzyme.
(canceled)
Dependencies are inferred from the claim text.