Systems and methods for meso-dissection
AssigneeRoche Sequencing Solutions, Inc.
InventorsMichael Barnes, Christophe Chefd'hotel, Srinivas Chukka, Mohammad Qadri
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Abstract
The subject disclosure presents systems and methods for improved meso-dissection of biological specimens and tissue slides including importing one or more reference slides with annotations, using inter-marker registration algorithms to automatically map the annotations to an image of a milling slide, and dissecting the annotated tissue from the selected regions in the milling slide for analysis, while concurrently tracking the data and analysis using unique identifiers such as bar codes.
Claims15
An instrument for meso-dissection, comprising:
- a processor;
- and
- a memory coupled to the processor, the memory to store computer-readable instructions that, when executed by the processor, cause the processor to perform operations comprising:
- importing a reference image along with one or more annotations, wherein the reference image was digitized from a reference slide scanned on a whole-slide scanner and wherein the annotations were generated using a whole slide viewer interface coupled to the whole-slide scanner;
- and
- automatically registering said one or more annotations onto a live capture of a tissue specimen slide to be milled;
- wherein tissue is extracted from the tissue specimen slide is dissected based on the one or more annotations, resulting in a milled tissue sample.
A tangible non-transitory computer-readable medium to store computer-readable code that is executed by a processor to perform operations comprising:
- generating a milling annotation for milling a tissue specimen based on a plurality of reference annotations;
- and
- milling the tissue specimen using the milling annotation,
- wherein the milling annotation is generated based on automatic registration of an annotation generated on an external whole-slide scanner on to a live capture of the tissue specimen.
A computer-implemented method for meso-dissection comprising the steps:
- generating a milling annotation for milling a tissue specimen based on a plurality of reference annotations;
- and
- milling the tissue specimen using the milling annotation,
- wherein the milling annotation is generated based on automatic registration of an annotation generated on an external whole-slide scanner on to a live capture of the tissue specimen.
Dependencies are inferred from the claim text.