Detection of nucleic acids from multiple types of human papillomavirus
AssigneeGen-Probe Incorporated
InventorsSylvia A. NORMAN, Jennifer J. BUNGO, William L. HANNA, Neeraj P. RAO
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Abstract
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
Claims20
A combination of oligomers for detecting human papillomavirus (HPV) types in group A2, the combination comprising:
- a first amplification oligomer comprising a target-specific sequence and a 5′ promoter sequence, wherein the first amplification oligomer target-specific sequence consists of SEQ ID NO:25 or SEQ ID NO:27, or an RNA equivalent thereof;
- and
- a second amplification oligomer comprising a target-specific sequence consisting of SEQ ID NO:38, or an RNA equivalent thereof.
A method for of determining the presence or absence of a human papillomavirus (HPV) nucleic acid type in group A2 in a sample, comprising the steps of:
- (a) contacting a sample, said sample suspected of containing a target nucleic acid of an HPV type in group A2, with a combination of amplification oligomers that amplify a target region of said target nucleic acid, wherein
- (i) a first amplification oligomer comprises a target-specific sequence and a 5′ promoter sequence, wherein the first amplification oligomer target-specific sequence consists of SEQ ID NO:25 or SEQ ID NO:27, or an RNA equivalent thereof;
- and
- (ii) a second amplification oligomer comprises a target-specific sequence consisting of SEQ ID NO:38, or an RNA equivalent thereof;
- (b) performing an amplification reaction using the amplification oligomers, wherein if the target nucleic acid of the HPV type in group A2 is present in the sample, an amplification product is produced;
- and
- (c) detecting the amplification product.
Dependencies are inferred from the claim text.