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US 2019/0376131 A1Published application
Compositions and methods for detection of hepatitis a virus nucleic acid
AssigneeGen-Probe Incorporated
InventorsJames D. CARLSON, Steven T. BRENTANO
Life of this patent
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Abstract
Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.
Claims24
A combination of at least two oligomers for amplifying a HAV target region comprising:
- a first oligomer comprising a target-specific sequence of about 24 to 28 nt contained in the sequence of SEQ ID NO:147 that includes at least the sequence of SEQ ID NO:148, and optionally joined at its 5′ end to a promoter sequence, and a second oligomer comprising a target-specific sequence of about 24 to 30 nt, contained in the sequence of SEQ ID NO:157 and that includes at least the sequence of SEQ ID NO:158.
-3. (canceled)
-8. (canceled)
A method of detecting the presence of HAV in a sample comprising the steps of:
- purifying HAV nucleic acid from other components in a sample containing HAV;
- amplifying a HAV target sequence in the purified HAV nucleic acid, or a cDNA made therefrom, by using an in vitro amplification reaction that includes at least two amplification oligomers specific for a selected HAV target region, which include:
- a first oligomer comprising a target-specific sequence of about 24 to 28 nt contained in the sequence of SEQ ID NO:147 that includes at least the sequence of SEQ ID NO:148, and optionally joined at its 5′ end to a promoter sequence, and a second oligomer comprising a target-specific sequence of about 24 to 30 nt contained in the sequence of SEQ ID NO:157 and that includes at least the sequence of SEQ ID NO:158;
- to produce an amplified product of the selected HAV target region;
- and
- detecting the amplified product.
3 more elements
-15. (canceled)
-19. (canceled)
Dependencies are inferred from the claim text.